POLECAMY
Koszyk
Genetic diversity of Scots pine (Pinus sylvestris L.) as an expression of adaptation to heavy industrial pollution: a case study of the population from Miasteczko Śląskie
Autor:
Format:
ibuk
The aim of the study was to explain why some Pinus sylvestris trees are more resistant to stress factors than others and whether the observed diversity results from natural selection or not. Two subpopulations of Scots pine were analyzed, both of which grew in an area which was severely polluted with heavy metals. Parental trees and seedlings were monitored for four consecutive years in the buffer zone of the zinc plant in Miasteczko Śląskie. The content of heavy metals in soil and plant material markedly exceeded threshold values, particularly for Zn, Pb and Cd. The morphological traits, isoenzymatic and microsatellite lod as well as cytological data were analyzed. The obtained results indicate that there are clear genetic differences between trees which are sensitive and those which are resistant to contamination in a naturally regenerated pine stand. These differences were recorded both in phenotypes (morphology, cytology, isoenzymes) and genotypes (microsatellite DNA). Adaptive genetic diversity observed in the two generations reflects differences in the survival capabilities of individuals exposed to stress and points to the selective pressure against trees with specific genotypes.
| Rok wydania | 2013 |
|---|---|
| Liczba stron | 132 |
| Kategoria | Genetyka |
| Wydawca | Wydawnictwo Naukowe Uniwersytetu im. Adama Mickiewicza |
| ISBN-13 | 978-83-232-2575-1 |
| Numer wydania | 1 |
| Język publikacji | polski |
| Informacja o sprzedawcy | ePWN sp. z o.o. |
Ciekawe propozycje
Spis treści
| 1. Introduction | 7 |
| 2. Literature review | 10 |
| 3. Study area and plant material | 20 |
| 3.1. Characteristics of study area | 21 |
| 3.1.1. Characteristics of soil properties | 21 |
| 3.1.2. Index of forest decline caused by heavy metal pollution | 22 |
| 3.2. Plant material | 23 |
| 3.2.1. Health condition of trees | 23 |
| 3.2.2. The heavy metal content in the needles | 25 |
| 3.3. Statistical analysis | 26 |
| 4. Response of Pinus sylvestris L. population to soil pollution from heavy metals expressed in the morphology and anatomy of needles | 27 |
| 4.1. Material and methods | 27 |
| 4.1.1. Morphology and anatomy | 27 |
| 4.1.2. Fluctuating asymmetry | 28 |
| 4.1.3. Data analysis | 29 |
| 4.2. Results | 29 |
| 4.2.1. Morphology and anatomy | 29 |
| 4.2.2. Fluctuating asymmetry | 41 |
| 5. Genetic structure of Pinus sylvestris L. population from Miasteczko Śląskie: isoenzymatic variation | 46 |
| 5.1. Material and methods | 46 |
| 5.1.1. Plant material | 46 |
| 5.1.2. Isozyme analysis | 46 |
| 5.1.3. Data analysis | 47 |
| 5.2. Results | 48 |
| 5.2.1. Allele and genotype frequencies | 48 |
| 5.2.2. Variability between populations | 53 |
| 6. Genetic structure of Pinus sylvestris L. population from Miasteczko Śląskie: nuclear microsatellite variation | 61 |
| 6.1. Material and methods | 61 |
| 6.1.1. Plant material | 61 |
| 6.1.2. DNA preparation and microsatellite analysis | 61 |
| 6.1.3. Data analysis | 62 |
| 6.2. Results | 63 |
| 6.2.1. Allele frequencies | 63 |
| 6.2.2. Genetic variability | 67 |
| 7. The influence of soil pollution on cytogenetic characteristics of Scots pine | 73 |
| 7.1. Material and methods | 73 |
| 7.1.1. Plant material | 73 |
| 7.1.2. Fixation and cytological analysis | 73 |
| 7.1.3. Data analysis | 74 |
| 7.2. Results | 74 |
| 8. Discussion | 82 |
| 8.1. Soil contamination | 82 |
| 8.2. Morphological and anatomical variation | 83 |
| 8.3. Isoenzymatic variation | 89 |
| 8.4. Microsatellite diversity | 95 |
| 8.5. Differentiation of cytological characteristics | 99 |
| General Conclusions | 104 |
| References | 108 |
| Appendix | 119 |
| Zróżnicowanie genetyczne sosny zwyczajnej (Pinus sylvestris L.) jako wyraz adaptacji do silnych zanieczyszczeń przemysłowych na przykładzie populacji z Miasteczka Śląskiego (Streszczenie) | 130 |
Wypożyczaj w abonamencie!
Już od 2,66 zł za ebooka
